Viral Replication Imaging Analysis

Viral Replication Imaging Analysis

Virus replication is a complex and highly coordinated process. After entering a cell, a virus undergoes several processes to multiply in order to produce more progeny viruses. Although the replication process varies greatly between virus types, all viral replication cycles involve the following steps: I) attachment to and entry into the host cell, followed by shedding of the capsid structure and release of the viral genome; II) replication of the genome and synthesis of viral particle components in the host cell; III) assembly and release of the offspring virus from the host cell. Real-time monitoring of viral replication is important for the suppression of virus transmission and the treatment of viral diseases. Therefore, imaging analysis of the viral replication process is a key concern for researchers.

Generic viral replication cycle and examples for results obtained on individual replication steps using site-specific chemical labeling approaches.Figure 1. Generic viral replication cycle (a–h) and examples for results obtained on individual replication steps using site-specific chemical labeling approaches (i–ix) (Sakin V, et al. 2016).

Viral Replication Imaging Analysis

In recent years, the study of viral replication has shifted from overall measurements to imaging analysis at the single-particle level. Traditional fluorescence microscopy techniques allow researchers to observe the details of viral and individual replication at a point in time to obtain detailed quantitative information. However, traditional imaging techniques can only provide a snapshot of virus replication, they cannot monitor the entire process of virus replication in real time.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can use VIRIM technology to monitor the replication of individual viruses in host cells in real time to study the heterogeneity of replication dynamics between cells.

Viral Replication Imaging Analysis Workflow

The following are the specific steps of viral replication imaging analysis

Cell line culture

Cell line culture

Step 1
Virus preparation

Virus preparation

Step 2
Viral replication imaging

Viral replication imaging

Step 3
Data analysis

Data analysis

Step 4

Delivery

Images for single-molecule imaging analysis
Single-cell dynamics and heterogeneity of viral replication
Mobility of translated RNA
The fluorescence intensity
Other data that you need

Our Advantages

  • Visual analysis of viral replication at the single-cell level
  • Real-time monitoring of virus replication
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of viral replication imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

References
  1. Boersma S, Rabouw H H, Bruurs L J M, et al. Translation and replication dynamics of single RNA viruses[J]. Cell, 2020, 183(7): 1930-1945. e23.
  2. Frensing T, Kupke S Y, Bachmann M, et al. Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells[J]. Applied microbiology and biotechnology, 2016, 100(16): 7181-7192.
  3. Sakin V, Paci G, Lemke E A, et al. Labeling of virus components for advanced, quantitative imaging analyses[J]. FEBS letters, 2016, 590(13): 1896-1914.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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