Bacteria Transcript Imaging Analysis

Bacteria Transcript Imaging Analysis

Transcription is a highly regulated process. In bacteria, only one type of RNA polymerase (RNAP) is responsible for the transcription of coding and non-coding RNA. Bacteria rely heavily on transcription and translation to produce proteins, which help them make specific responses to environmental changes. Therefore, visual analysis of bacterial transcription process is very important to study the interaction between protein and nucleic acid.

Transcription cycleFigure 1. Transcription cycle (Stracy M, et al. 2017).

Bacteria Transcript Imaging Analysis

Currently, multiple analysis methods are available for transcription. In vitro single-molecule experiments can directly observe the behavior of individual molecules to determine the dynamics of their interactions. Chromatin immunoprecipitation can be used to determine the specific binding sequence of the target protein and nucleic acid through the lysate from the cell population. However, these techniques cannot analyze the spatial information of cell transcription or the dynamics involved.

CD BioSciences has been committed to the development of imaging technology for many years. We can observe the transcription mechanism of bacteria through in vivo single-molecule and super-resolution technology, revealing the binding kinetics of the proteins involved in the process.

Bacteria Transcript Imaging Analysis Workflow

The following are the specific steps of bacteria transcript imaging analysis:

Preparation of fluorescent fusion strains

Preparation of fluorescent fusion strains

Step 1
Sample preparation

Sample preparation

Step 2
Super-resolution imaging

Super-resolution imaging

Step 3
Quantitative imaging and data analysis

Quantitative imaging and data analysis

Step 4

Delivery

Super-resolution images of bacterial transcription
Analyze single particle tracking PALM data
Spatial organization of transcription and non-specific DNA interaction
Diffusion coefficient of freely diffusing non-conjugated fluorescent protein
Proportion of RNAP transcribed genes
Provide other data according to your needs

Our Advantages

  • Minimize unwanted background fluorescence
  • Super-resolution imaging and tracking of RNA polymerase in live bacteria
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacteria transcript imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

References
  1. Stracy M, Kapanidis A N. Single-molecule and super-resolution imaging of transcription in living bacteria[J]. Methods, 2017, 120: 103-114.
  2. Stracy M, Uphoff S, de Leon F G, et al. In vivo single-molecule imaging of bacterial DNA replication, transcription, and repair[J]. FEBS letters, 2014, 588(19): 3585-3594.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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