Imaging Flow Cytometry
Flow cytometry is a powerful technique widely used in cell counting, biomarker detection, and cell sorting. However, it has low information content and cannot measure the morphology and spatial information of a single cell. Compared with traditional flow cytometry, imaging flow cytometry increases the number of parameters that can be measured by providing cell morphology and spatial information in a high-throughput and controllable manner. Therefore, it is widely used in biological disciplines, such as immunology, cell biology, and microbiology.
Figure 1．Imaging flow cytometry acquires images of single cells in high throughput (Doan, et al., 2018).
Imaging flow cytometry (IFC) is a powerful tool that combines flow cytometry with digital microscopes, allowing us to collect information from individual cells to generate quantitative high-throughput imaging data. CD BioSciences uses imaging flow cytometry to provide you with a series of imaging analysis services such as cell counting, cell apoptosis, and cell proliferation. If you have any needs, please feel free to contact us.
Summary of Imaging Flow Cytometry (IFC) Techniques
|Features||ImageStream||Multiple-view||Temporally Coded Excitation||STEAM||FIRE||Spatial–temporal Transformation|
|Illumination (spectral range)||Laser||LED||Laser||Pulse laser||Laser||Laser|
|Detector||TDI CCD||CMOS||CMOS||Single-pixel detector||PMT||PMT|
|Data transfer & computation complexity||Simple||Simple||Simple||Complex||Complex||Simple|
|Inherit functions from FC||Yes||No||Yes||No||Yes||Yes|
|Compatibility with sorting||No||No||No||No||No||Yes|
|Throughput (cells per s)||Up to 1000||∼10000||∼2000||Up to 100000||∼50000||>1000|
|Real-time processing solution||Yes||No||No||Yes||No||Yes|
FC: conventional flow cytometry.
Application of Imaging Flow Cytometry
Co-localization of two proteins
Changes in morphological changes
Immune synapse formation
Cell cycle analysis
Cell proliferation analysis
Cell viability analysis
Binding of two cells
Cell autophagy analysis
Advantages of Imaging Flow Cytometry
- High fluorescence sensitivity
- High image resolution capability
- High-speed processing
- Ability to analyze changes in cell or nuclear morphology
- Rare cell detection capability
- The ability to understand the interaction between cells
- Doan, Minh, et al. "Diagnostic potential of imaging flow cytometry." Trends in biotechnology 36.7 (2018): 649-652.
- Han, Yuanyuan, et al. "Imaging technologies for flow cytometry." Lab on a Chip 16.24 (2016): 4639-4647.
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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!