Protein Phase Separation Imaging Services

Protein Phase Separation Imaging Services

In addition to the classic membrane-bound organelles, cells can also perform biochemical reactions in non-membrane compartments. These non-membrane compartments are liquid-like structures formed by proteins through the liquid-liquid phase separation (LLPS) process, which can perform a variety of important biological functions. Most proteins involved in phase separation contain low-complexity regions or natural disordered regions, and abnormally phase-separated of proteins can cause many diseases. Therefore, the visual analysis of the protein phase separation process is very important for the research of a variety of human diseases.

Mechanism of protein phase separationFigure 1. Mechanism of protein phase separation (Wen J, et al., 2021).

Protein Phase Separation Imaging Analysis

In recent years, protein phase separation has become a new research hotspot in the field of biology. Single-molecule fluorescence technology is a powerful tool for studying the conformation and dynamics of natural disordered proteins, which promotes the understanding of protein folding, allosteric, assembly dynamics, and its relationship with function at the single-molecule level. At the same time, this technology can also be used to visually study the potential molecular mechanisms of protein liquid-liquid and liquid-solid phase transitions.

CD BioSciences has been committed to the research and development of fluorescent probes and imaging technology for many years. We can use single-molecule fluorescence imaging technology and fluorescence correlation spectroscopy to study the intramolecular conformational changes and intermolecular interactions during protein phase separation to help you reveal the mechanism of protein phase separation at the single-molecule level.

Protein Phase Separation Imaging Analysis Workflow

The following are the specific steps of protein phase separation imaging analysis:

Protein expression and purification

Protein expression and purification

Step 1
Fluorescent labeling of protein mutants

Fluorescent labeling of protein mutants

Step 2
Protein phase separation and detection

Protein phase separation and detection

Step 3
Single molecule fluorescence imaging

Single molecule fluorescence imaging

Step 4
Data processing and analysis

Data processing and analysis

Step 5


Image of protein phase separation process
Conformational changes of proteins during phase separation
Interaction between protein molecules
The morphology of protein aggregates
Other data that you need

Our Advantages

  • High fluorescence efficiency and stable fluorescence
  • Fluorescent molecules are small and do not destroy the biological activity of the target molecule
  • It can track the changes of individual molecules or molecular complexes in real time at the nanometer scale
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of protein phase separation imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

  1. Pan Q, Sun D, Xue J, et al. Real-time study of protein phase separation with spatiotemporal analysis of single-nanoparticle trajectories[J]. ACS nano, 15(1):539-549.
  2. Wen J, Hong L, et al. Conformational expansion of tau in condensates promotes irreversible aggregation[J]. American Chemical Society 143(33), 13056-13064.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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