Bacterial Viability Imaging Analysis

Bacterial Viability Imaging Analysis

Bacterial viability detection has been widely used in the fields of biotechnology, food industry, medicine, and environmental monitoring. It has become an important indicator for evaluating antibacterial properties and monitoring microbial viability in water. Traditionally, nucleic acid staining, membrane potential, and redox indicators are often used to measure cell status indirectly to assess bacterial viability. But these methods cannot directly observe the growth and division of bacteria. In recent years, with the development of fluorescent probes and imaging technology, researchers can directly observe the morphology and growth of bacteria through microscope imaging, and then evaluate the bacterial activity.

Bacillus gram positive stain under the microscope view.Figure 1. Bacillus gram positive stain under the microscope view.

Bacterial Viability Imaging Analysis

Due to its rapidity and the powerful ability to analyze complex samples, flow cytometry has become a popular tool for microbiology research. However, traditional flow cytometry cannot observe the morphology of each bacterial cell. With the advent of imaging flow cytometry, researchers have gained new insights into microbial phenomena that cannot be explored by traditional techniques.

CD BioSciences has been committed to the development of fluorescent probes and imaging technology for many years. We can use imaging flow cytometry to capture a clear image of each bacterial cell, and then analyze its morphological characteristics to evaluate the viability of the bacterial cell.

Bacterial Viability Imaging Analysis Workflow

The following are the specific steps of bacteria viability imaging analysis:

Instrument setup and calibration

Instrument setup and calibration

Step 1
Bacteria suspension preparation and staining

Bacteria suspension preparation and staining

Step 2


Step 3
Data processing and analysis

Data processing and analysis

Step 4


Number of live bacteria
Total number of bacteria
Other data that you need

Number of dead bacteria
Raw data of bacteria images

Our Advantages

  • Reliably and accurately determine the concentration of bacterial cells
  • Visually analyze the morphological characteristics of live and dead cells
  • High-throughput imaging analysis, which can quickly analyze multiple samples
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacteria viability imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  


  1. Stiefel P, Schmidt-Emrich S, Maniura-Weber K, et al. Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide[J]. BMC microbiology, 2015, 15(1): 1-9.
  2. Pan Y, Kaatz L. Use of image‐based flow cytometry in bacterial viability analysis using fluorescent probes[J]. Current protocols in microbiology, 2012, 27(1): 2C. 5.1-2C. 5.11.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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