Lysosomes Imaging Services
The lysosome is a small membrane-enclosed cytoplasmic organelle with a size ranging from 100 to 500 nm. Lysosomes are the center of cell catabolism, which contain hydrolytic enzymes for the metabolism of a variety of biomolecules. Extracellular substances are degraded by lysosomes after being transported to membrane-bound endosomes, while cellular substances are transported to lysosomes through autophagy. Lysosomal dysfunction may induce a variety of diseases, such as aging and neurodegenerative diseases. Therefore, visual tracking of lysosomal dynamics is very important to deeply understand the working principle of lysosomes.
Figure 1. The structure of lysosomes.
Lysosomes Imaging Analysis
Optical microscopy and molecular fluorophores are popular tools for imaging lysosomal morphology and localization, and studying lysosome-related cellular activities in living cells. However, traditional optical microscopes have limited resolution. Therefore, super-resolution microscopes have become an effective tool for monitoring the working mechanism of lysosomes in cell biological processes. CD BioSciences can develop lysosome-specific fluorescent probes for you to monitor lysosomes in real time and determine the spatial distribution, molecular mechanisms and cellular functions of lysosomes in cells.
Lysosomes Imaging Analysis Workflow
The following are the specific experimental steps of lysosomes imaging analysis:
Synthetic fluorescent probe
We will design and synthesize fluorescent probes for you according to your needs, and characterize them by NMR, dynamic light scattering and GC.
Step 1
Cell culture
The cells were seeded in DMEM and incubated for a period of time at 37°C in a 5% CO2 environment. Then remove the cell culture medium and incubate with fresh culture medium.
Step 2
Cell staining
The cells were seeded into a 16-well culture plate. After a period of incubation, the cells are treated with stain for 30 minutes. Then wash with PBS and maintain the cells in the cell culture medium.
Step 3
Confocal fluorescence imaging
The processed cells are imaged by super-resolution microscopy to monitor the morphological changes of lysosomes during cellular events, and to obtain images at different time points.
Step 4
Image analysis
After the original image is corrected by computer image analysis software, we will analyze the image according to your needs and obtain the relevant data analysis report.
Step 5Delivery
Fluorescent probes and their characterization data
Number and location of lysosomal organelles
Lysosomal enzyme activity
Image analysis report
The area of each lysosome in the cell
Fluctuations in lysosome pH
The original image
Other relevant data
Our Advantages
- High resolution image analysis
- Low background fluorescence, high signal-to-noise ratio
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has a professional team and advanced imaging equipment. The entire process of lysosomes imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
- Yadav A, Rao C, Nandi C K. Fluorescent probes for super-resolution microscopy of lysosomes[J]. ACS omega, 2020, 5(42): 26967-26977.
- Ba Q, Raghavan G, Kiselyov K, et al. Whole-cell scale dynamic organization of lysosomes revealed by spatial statistical analysis[J]. Cell reports, 2018, 23(12): 3591-3606.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!