Lysosomes Imaging Analysis
The lysosome is a small membrane-enclosed cytoplasmic organelle with a size ranging from 100 to 500 nm. Lysosomes are the center of cell catabolism, which contain hydrolytic enzymes for the metabolism of a variety of biomolecules. Extracellular substances are degraded by lysosomes after being transported to membrane-bound endosomes, while cellular substances are transported to lysosomes through autophagy. Lysosomal dysfunction may induce a variety of diseases, such as aging and neurodegenerative diseases. Therefore, visual tracking of lysosomal dynamics is very important to deeply understand the working principle of lysosomes.
Figure 1. The structure of lysosomes.
Lysosomes Imaging Analysis
Optical microscopy and molecular fluorophores are popular tools for imaging lysosomal morphology and localization, and studying lysosome-related cellular activities in living cells. However, traditional optical microscopes have limited resolution. Therefore, super-resolution microscopes have become an effective tool for monitoring the working mechanism of lysosomes in cell biological processes. CD BioSciences can develop lysosome-specific fluorescent probes for you to monitor lysosomes in real time and determine the spatial distribution, molecular mechanisms and cellular functions of lysosomes in cells.
Lysosomes Imaging Analysis Workflow
The following are the specific experimental steps of lysosomes imaging analysis:
Fluorescent probes and their characterization data
Number and location of lysosomal organelles
Lysosomal enzyme activity
Image analysis report
The area of each lysosome in the cell
Fluctuations in lysosome pH
The original image
Other relevant data
- High resolution image analysis
- Low background fluorescence, high signal-to-noise ratio
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has a professional team and advanced imaging equipment. The entire process of lysosomes imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
- Yadav A, Rao C, Nandi C K. Fluorescent probes for super-resolution microscopy of lysosomes[J]. ACS omega, 2020, 5(42): 26967-26977.
- Ba Q, Raghavan G, Kiselyov K, et al. Whole-cell scale dynamic organization of lysosomes revealed by spatial statistical analysis[J]. Cell reports, 2018, 23(12): 3591-3606.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!