Muscle Tissue Morphology Imaging Services

Muscle is a tissue composed of specialized cells or fibers that are capable of contracting to enable movement. Accurately assessing and quantifying the morphology of musculoskeletal tissue is critical to understanding human physiology and disease states. However, traditional 2D imaging techniques have limitations that do not allow for a comprehensive presentation of the 3D morphology and structural features of muscle tissue.

Fig. 1 Types of muscle tissue.

Services Overview

CD BioSciences utilizes 3D imaging and histology techniques combined with animal models that allow us to provide our clients with morphological imaging analysis of muscle tissue. We are able to fully present the morphology of muscle tissue and analyze it quantitatively. In addition, we specialize in the study of the connection mechanisms between nerves and muscles, which provides important support for the understanding of human physiology and disease states.

Muscle Tissue Morphology Imaging Services

Muscle tissue 3D ultrasound imaging analysis services

3D Ultrasound Imaging technology is a specific 3D imaging technique that utilizes ultrasound waves to obtain a three-dimensional image of an object. CD BioSciences utilizes 3DUS technology to provide our clients with muscle tissue morphology imaging analysis services. Our services include:

• Help clients perform morphometric analysis of musculoskeletal tissue morphology.
• Quantify 3D morphological characteristics of skeletal muscle, which include:
  • Muscle tissue size: measure the volume or mass of muscle tissue.
  • Cluster length: measure how far the muscle fibers extend in 3D space.
  • Angle of inclination: analyze the angle of inclination of the muscle fibers or tissue.

MEP imaging analysis services

Motor endplates (MEP) are critical structural and functional interfaces between motor neurons and skeletal muscle, responsible for receiving electrical signals from motor neurons and generating endplate potentials that trigger muscle contraction. However, MEPs are not uniformly distributed in skeletal muscle, and thus local structural information cannot accurately reflect the status of MEPs in the entire skeletal muscle. To fully understand the spatial distribution of MEPs in skeletal muscle, we need to obtain their 3D distribution at the overall level.

CD BioSciences utilizes tissue clearing technology and light-sheet fluorescence microscopy to provide our clients with muscle tissue morphology imaging analysis services, focusing on the spatial distribution and structure of MEPs in skeletal muscle. The following are our specific services:

3D spatial distribution analysis

CD BioSciences labels motor endplates in whole skeletal muscle by utilizing injections of α-BTX, combined with tissue transparency techniques and light sheet microscopy. Through our imaging analysis, clients can obtain:

• Spatial distribution of MEPs throughout skeletal muscle.
• 3D distribution maps and morpho-structural information of MEPs in different skeletal muscles.

Spatial imaging analysis of neuromuscular

CD Bioscience can help clients explore the relationship between the spatial distribution of MEPs and muscle tissue. Our services mainly include:

• Observe the morphological spatial distribution of MEPs and nerve branches within muscles to reveal the connections between nerves and muscles.
• Utilize mouse models such as the sciatic nerve dissection model to observe changes in muscle atrophy and MEPs.
• Study the changes of skeletal muscle MEPs after peripheral nerve injury and repair.
• Stain the MEP at different time points after innervation with α-BTX to observe the spatial conformation of AChRs and assess the effect of nerve injury on MEP.

CD BioSciences has a professional team and advanced imaging equipment. The whole process of muscle tissue morphology imaging services is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!