Immune Cell Infiltration Imaging Analysis

Immune Cell Infiltration Imaging Analysis

Tumor immune cell infiltration refers to the migration of immune cells from the blood to tumor tissues and begin to play its role. Different immune cells play different roles in the tumorigenesis process, and the immune cell composition of different tumors also has its own characteristics. The infiltration of immune cells in tumors is closely related to clinical results. Immune cells infiltrated in tumors are most likely to be used as drug targets to improve the survival rate of patients. Therefore, the quantitative analysis of tumor immune cell infiltration is very important.

Immunohistochemical features of tumour-infiltrating immune or inflammatory cellsFigure 1. Immunohistochemical features of tumor-infiltrating immune/inflammatory cells (Ino, Y., et al. 2013).

Immune Cell Infiltration Imaging Analysis

The traditional in vitro assay of immune cell infiltration monitors the interaction between treatment and immune cells in the form of 2D assay. These models cannot replicate the tissue microenvironment, so they cannot fully simulate the in vivo conditions of immune cell infiltration research. The 3D cell culture model provides similar in vivo tissue complexity and is ideal for evaluating the enhancement of immune cell infiltration after treatment with your compound. CD BioSciences uses functional markers to perform high-throughput imaging analysis of tumor spheres to quantify infiltration over time to ensure consistency of measurement and reliability of data.

Requirements and Workflow of Immune Cell Infiltration Imaging Analysis

Method High content imaging
Cell model 3D cell models (e.g. tumor spheroids, organoids, etc.)
Available cell Cancer cell lines, primary cells, cancer stem cells, client provided cells
Quality controls        Vehicle control

The following are the specific experimental steps of the immune cell infiltration imaging analysis:

Immune Cell Infiltration Imaging Analysis

Eight main steps transform images into quantitative information to support experimental conclusions.

3D cell models, grown to approximately 200 μm in diameter
Treatment with test compounds
Seeding PBMCs at 4000 cells/well
Fixation after 24 hours
Images are analyzed to quantify total number of infiltrating immune cells
High content imaging is conducted on well plates
Tissue clearing is applied to render 3D cell models transparent
Nuclei are labeled with DAPI


Dose-response curve between the number of infiltrating T cells and the dose of test compound
Total cell count for each treatment group
Immune cell infiltration distance distribution
Assess the statistical significance of the results regarding the vehicle and treatment groups

Our Advantages

  • High-sensitivity optical system to capture high-quality pictures
  • Fully automatic image analysis and data management
  • Unbiased imaging analysis from single layer to the sphere
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of immune cell infiltration imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with personalized immune cell infiltration imaging analysis services according to your needs.

  1. Ino, Y., et al. "Immune cell infiltration as an indicator of the immune microenvironment of pancreatic cancer." British journal of cancer 108.4 (2013): 914-923.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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