DNA Structure Imaging Analysis
DNA is an abbreviation for deoxyribonucleic acid, which is present in all prokaryotic and eukaryotic cells and many viruses. Single-stranded DNA molecules can be assembled into highly complex 1D, 2D and 3D superstructures with specific functions. In the cell, DNA and protein assemble into chromosomes. Real-time study of DNA structure and DNA-protein interaction at the cellular level requires the most advanced biological imaging technology.
Figure 1. 3D super-resolution imaging of metaphase chromosomes (Yardimci, et al. 2020).
DNA Structure Imaging Analysis
Since Franklin revealed the double helix structure of DNA, a series of biophysical techniques such as X-ray crystallography, electron microscopy, and nuclear magnetic resonance have been used to characterize DNA structure. However, these technologies are limited by spatial resolution and cannot perform high-resolution studies on the local helical structure and mechanics of DNA molecules. Atomic force microscope is an effective imaging tool, which can image and characterize the structure of a single biomolecule under physiological conditions.
CD BioSciences has been committed to the research and development of fluorescent probes and imaging technology for many years. We can image and analyze the secondary and tertiary structures of DNA by atomic force microscope to study the intramolecular variation between the depth of grooves in DNA molecules and the interaction between protein and DNA.
DNA Structure Imaging Analysis Workflow
The following are the specific steps of DNA structure imaging analysis:
Fill in the online form to submit your requirements for DNA structure imaging analysis.
Send us the DNA that need to be imaged and analyzed.
We use atomic force microscope to study the secondary and tertiary structures of DNA and the interaction between protein and DNA.
After the experiment is completed, we will send the sample and experimental data to you.
Adsorption degree of DNA in solution
The secondary and tertiary structure of DNA
Provide other data according to your needs
- High signal-to-noise ratio, high resolution
- Able to perform real-time dynamic imaging in physiologically relevant solutions
- No need for overall averaging or sample marking
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has a professional team and advanced imaging equipment. The entire process of DNA structure imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
- Main, Kavit HS, et al. "Atomic force microscopy—A tool for structural and translational DNA research." APL bioengineering 5.3 (2021): 031504.
- Yardimci, Sevim, et al. "Three-dimensional super-resolution fluorescence imaging of DNA." Scientific reports 10.1 (2020): 1-8.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!