Real-time Imaging Analysis of Cytotoxicity
Cytotoxicity refers to the ability of some chemicals or mediators to destroy living cells. The use of cytotoxic compounds can induce necrosis or apoptosis of healthy living cells. Therefore, accurate measurement of cytotoxicity is very important in the new drug R & D stage. By analyzing the cytotoxicity level, we can measure the health risk caused by new drugs to the human body.
Figure 1. Real-time imaging detection of cytotoxicity.
Real-time Imaging Analysis of Cytotoxicity
Cytotoxicity is generally detected by the measurement of living dyes excluded by healthy cells, markers released by dead cells and metabolic activity. However, none of these methods can directly count the number of dead cells over time. CD BioSciences can provide you with one-stop cytotoxicity detection and analysis service. Our professional imaging analysis system allows you to observe cell death and measure cytotoxicity at any time.
Cytotoxicity Analysis Workflow
The live cell analysis system of CD BioSciences can perform real-time, automated cytotoxicity determination in tissue culture incubator. When the fluorescent dye is added to the cell culture medium, it is inert, non-fluorescent and will not enter living cells. With cell death and loss of membrane integrity, fluorescent dye will enter the cell and fluorescently label the nucleus. Over time, fluorescently labeled cell nuclei will be revealed, and then dying cells can be identified and quantified through high-definition images, and cytotoxicity can be analyzed. This method is suitable for eukaryotic cells, but not for yeast or bacteria. The detailed steps are as follows:
Step 1
Experiment plan
Design your experiment, considering the instruments and reagents required for each step.
Step 2
Cell culture
Let cells grow under the most suitable conditions.
Step 3
Cell marker
Design your experiment, considering the instruments and reagents required for each step.
Step 4
Acquire cell images
Capture target cell images with maximum clarity and resolution.
Step 5
Cytotoxicity analysis
Use the cell imaging analysis software to analyze the cytotoxicity.
Our Advantages
- Use time-lapse imaging to visualize and quantify cytotoxicity
- Automatically analyze the time course of cell death in the incubator
- The experimental results are accurate and repeatable
- Experienced scientists provide experimental consultation
- Reasonable price
CD BioSciences has a professional team and advanced imaging equipment. The entire process of cytotoxicity imaging assays is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs for cytotoxicity imaging analysis, please feel free to contact us. We can customize a personalized experimental program for you according to your project.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!