Imaging-based Analysis of Cellular Transfection Efficiency

Imaging-based Analysis of Cellular Transfection Efficiency

Transfection is a method of introducing exogenous nucleic acid into cells to produce genetically modified cells. It is used by researchers to study the function and regulation of genes. In addition, transfection can also be used to identify protein functions. Transfection efficiency is the proportion of cells that have obtained foreign elements in a sample, and it can be evaluated using a variety of different methods. Among them, automated imaging and analysis tools are effective and robust methods for measuring transfection efficiency.

Representative overlays of various transfection ratesFigure 1. Representative overlays of various transfection rates (Oberdanner C B, et al. 2019).

Imaging-Based Analysis of Transfection Efficiency

Time-lapse imaging of live cells is a powerful method for monitoring the transfection process at any time. Compared with transfection efficiency analysis methods such as Western blotting, flow cytometry, or real-time qPCR, live cell imaging can provide a more direct way to evaluate the efficiency of cell transfection, and can quickly provide reliable results, while requiring minimal cost.

Imaging-Based Analysis of Transfection Efficiency

Imaging-Based Analysis of Transfection Efficiency Workflow

CD BioSciences combines bright field and fluorescence imaging to quickly and directly quantify cell viability and transfection efficiency. It is non-invasive, automated, and avoids the use of nucleic acid stains. Therefore, the imaged samples can also be used in multiple downstream applications. The following is the workflow of imaging-based analysis of transfection efficiency.

Cell culture

Culture the GFP-transfected target cells in a medium supplemented with 10% FBS in a humid environment at 37°C.

Sample preparation

Inoculate transfected and untransfected cells in different mixing ratios into 96-well tissue culture plates, and then allow the cells to adhere overnight.

Treatment of cells

Samples with different transfection efficiencies were stained with a stain at room temperature for 30 minutes.

Image acquisition and analysis

Image collection is performed on the processed cells, and the collected data is analyzed with image analysis software.

Delivery

Experiment setting parameters
The original image
GFP transfection efficiency
Other relevant data

Our Advantages

  • Real-time monitoring of cell fusion and cell health
  • Monitor the intensity of expression
  • High-throughput detection, simple and cost-effective
  • Reducing data discrepancies caused by operations
  • Experienced scientists provide experimental consultation

CD BioSciences has a professional team and advanced imaging equipment. The entire process of transfection efficiency analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us, we can provide you with high-quality image-based transfection efficiency analysis services.

Reference
  1. Oberdanner C B, Eggenhofer N. Imaging-based analysis of cellular transfection efficiency. 2019.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

Online Inquiry