Amino Acid Metabolism Imaging Analysis

Amino Acid Metabolism Imaging Analysis

The amino acids produced by protein degradation in vivo and the non-essential amino acids synthesized in vivo are endogenous amino acids, while the amino acids digested and absorbed by food proteins are exogenous amino acids, which are distributed throughout the body to form an amino acid metabolism library. Amino acids mainly exist in blood and tissues in the body. The amino acids digested and absorbed by food proteins first enter the blood and then enter the tissue for biotransformation or decomposition. Tissue proteins can also decompose to produce amino acids into the blood. Abnormal metabolism of amino acids can lead to many diseases, such as cancer, diabetes and cardiovascular diseases. Therefore, imaging analysis of the metabolic process of amino acids is very important for the diagnosis and treatment of diseases.

Construction of glutamate fluorescence sensorFigure 1. Construction of glutamate fluorescence sensor (Marvin, et al. 2013).

Amino Acid Metabolism Imaging Analysis

Amino acid metabolism plays an important role in many physiological and pathological processes. In recent years, with the development of new fluorescent probes, researchers have realized fluorescence imaging of amino acid metabolism at the cellular level, and fluorescent probes have become an effective tool for visual analysis of amino acid metabolism.

CD BioSciences is a biotechnology company, which has been committed to the research and development of fluorescent probes and live cell imaging technology for many years. We can provide you with fluorescent probes for visual analysis of different amino acid metabolism to help you study the process of abnormal amino acid metabolism and the pathogenesis of related diseases.

Amino Acid Metabolism Imaging Analysis Workflow

The following are the specific steps of amino acid metabolism imaging analysis:

Synthetic fluorescent probes

Synthetic fluorescent probes

Step 1
Cell culture

Cell culture

Step 2
Fluorescence imaging

Fluorescence imaging

Step 3
Image processing and data analysis

Image processing and data analysis

Step 4


1H NMR, 13C NMR and mass spectra of fluorescent probes
Cytotoxicity of fluorescent probes
Spectral response of fluorescent probes to different concentrations of amino acids
Fluorescence images at different times
Provide other data according to your needs

Our Advantages

  • Real-time monitoring of amino acids metabolism at the cell level
  • High-throughput imaging analysis
  • Good cell membrane permeability and low cytotoxicity
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of amino acid imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with high-quality services that meet your needs.

  1. Yue Y, Huo F, Ning P, et al. Dual-site fluorescent probe for visualizing the metabolism of Cys in living cells[J]. Journal of the American Chemical Society, 2017, 139(8): 3181-3185.
  2. Marvin J S, Borghuis B G, Tian L, et al. An optimized fluorescent probe for visualizing glutamate neurotransmission[J]. Nature methods, 2013, 10(2): 162-170.
  3. Yang X, Liu W, Tang J, et al. A multi-signal mitochondria-targeted fluorescent probe for real-time visualization of cysteine metabolism in living cells and animals[J]. Chemical Communications, 2018, 54(81): 11387-11390.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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