Bacterial Count Based on Image

Bacterial Count Based on Image

Microbiological analysis usually depends on the accurate count of colony forming units (CFU). Colony counting generally spreads an equal amount of fully diluted liquid culture on a culture plate and cultivates it. After the selected microorganisms are incubated under appropriate conditions, the colonies are counted to determine the number of CFU. This process is based on the assumption that each colony is produced from a single bacterium and is completed by manually counting colonies.

Hand counting of bacterial colonies.Figure 1. Hand counting of bacterial colonies.

Bacterial Count Based on Image

Although manual counting is still the gold standard for colony counting, the process is time-consuming and prone to errors. Therefore, it is necessary to develop an image-based automatic colony counting method. CD BioSciences has been committed to the development of imaging technology for many years. We can provide you with a method for detecting and automatically calculating colonies based on processed images, which not only shows good performance in the accuracy of colony counting, but also shortens the time of colony counting.

The Workflow of Bacterial Count Based on Image

The following are the specific steps of bacterial count based on image:

Collection of microbiological samples

Collection of microbiological samples

Step 1
Sample preparation and dilution

Sample preparation and dilution

Step 2
Bacterial culture

Bacterial culture

Step 3
Imaging and counting

Imaging and counting

Step 4


High resolution images of colonies
Total number of colonies
Linear regression analysis between automatic colony count and gold standard
Provide other data according to your needs

Our Advantages

  • A more accurate and time-saving method for CFU determination
  • No need to use specific markers to highlight each colony
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacterial count based on image is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with high-quality services according to your needs.

  1. Kis B, Unay M, Ekimci G D, et al. Counting bacteria colonies based on image processing methods[C]//2019 Medical Technologies Congress (TIPTEKNO). IEEE, 2019: 1-4.
  2. Martinez-Espinosa J C, Cordova-Fraga T, Vargas-Luna M, et al. Nondestructive technique for bacterial count based on image processing[J]. Image, 2016, 12: 15.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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