Herpes Simplex Virus Infection Imaging Analysis

Herpes Simplex Virus Infection Imaging Analysis

Herpes simplex virus is a widespread human pathogen, which is infected by about 80% of adults. It can cause recurrent skin, oral cavity, lip, eye and genital herpes, locally manifested as painful vesicles filled with liquid. Herpes simplex virus infection shows a high degree of intercellular variability at the single-cell level, which affects the infection process to a great extent. Therefore, it is very important to visually analyze the infection process of herpes simplex virus.

Stages of Herpes on the lips with a description of disease.Figure 1. Stages of Herpes on the lips with a description of disease.

Herpes Simplex Virus Infection Imaging Analysis

Direct immunofluorescence is a method for identifying different proteins on the surface of the virus. It only requires one binding step, is the primary antibody is directly bound to the fluorescent label. The simplicity of direct immunofluorescence makes it a useful tool for initial disease diagnosis and monitoring treatment effects. In addition, it can be used for imaging analysis and quantification of virus content, including its intracellular location and its effect on cell morphology.

CD BioSciences has been committed to the research and development of fluorescent dyes and live cell imaging technology for many years. We will use digital wide-field microscopy and direct immunofluorescence to provide you with imaging analysis services for the infection process of herpes simplex virus.

Herpes Simplex Virus Infection Imaging Analysis Workflow

The following are the specific steps of herpes simplex virus infection imaging analysis:

Construction and cultivation of herpes simplex virus strain

Construction and cultivation of herpes simplex virus strain

Step 1
HSV-1 titration and infection

HSV-1 titration and infection

Step 2
Cell plating and infection

Cell plating and infection

Step 3
Image capture and data analysis

Image capture and data analysis

Step 4

Delivery

Cell images collected at different time points
Proportion of infected cells
The location of the virus contents in the cell
Provide other data according to your needs

Our Advantages

  • Real-time monitoring of the infection process
  • Automate image capture, processing and analysis
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of herpes simplex virus infection imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

Reference
  1. Drayman N, Karin O, Mayo A, et al. Dynamic proteomics of herpes simplex virus infection[J]. MBio, 2017, 8(6): e01612-17.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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