High Content Imaging
High-content imaging (HCI), also known as high-content screening (HCS), combines high-throughput automated imaging and analysis. It uses automated microscopy, fluorescence detection, and multi-parameter algorithms to visualize and image cellular events. High-content imaging was originally developed as a complementary technology to traditional biochemical high-throughput screening (HTS) in drug discovery, but with the continuous development and improvement of high-content imaging systems, it has been widely used in the field of life sciences.
Figure 1．The role of HCS technology in the transformation of biology into a large-scale, information-based science (Zanella, et al., 2010).
High-content imaging is a powerful tool for drug discovery. In addition to drug discovery, it can also be used to study cell morphology, cell proliferation, cell apoptosis, drug safety, and toxicity. CD BioSciences relies on high-throughput imaging technology, image analysis algorithm design and rich experience in biology to describe the cell physiology in complex cell systems and provide predictive and translatable data. If you have any needs, please feel free to contact us.
Available HCS Instrumentation
|Company||Brand name||Light source||Kinetic HCS||Opticsa|
|BD Biosciences||BD Pathway 855||Arc lamp||+||WF/CF|
|BD Biosciences||BD Pathway 435||Arc lamp||﹣||WF/CF|
|GE Healthcare||IN Cell Analyzer 2000||Arc lamp||+||WF|
|Intelligent Imaging Innovations||3i Marianas||Arc lamp||+(optional)||WF|
|Leica Microsystems||TCS SP5||Laser||+ (optional)||CF|
|Molecular Devices||ImageXpressMICRO||Arc lamp||+ (optional)||WF|
|Olympus||Scan^R||Arc lamp||+ (optional)||WF|
|Perkin Elmer||Operetta||Arc lamp||﹣||WF/CF|
|Thermo Scientific||Cellomics ArrayScan VTI||Arc lamp||+||WF/CF|
|Thermo Scientific||CellWoRx||Arc lamp||﹣||WF|
|TTP LabTech||Acumen eX3||Laser||﹣||WF|
a Abbreviations: CF, confocal microscope; WF, wide field.
Available HCS Informatics Tools
|Image processinga||BioImageXD||Open source|
|Database platforms||MySQL||Open source|
|Workflow systems||HCDC||Open source|
a Image analysis software which is integrated into commercial HCS platforms, such as Acapella (PerkinElmer), Attovision (BD Biosciences), IN Cell Investigator (GE Healthcare), and MetaXpress (Molecular Devices), is not listed here.
Application of High Content Imaging
Cell motility and migration
Receptor internalization and degradation
Translocation of transcription factors
Autophagy and protein aggregation
Protein acetylation and phosphorylation
Personal custom imaging assays
- Zanella, Fabian, James B. Lorens, and Wolfgang Link. "High content screening: seeing is believing." Trends in biotechnology 28.5 (2010): 237-245.
- Buchser, William, et al. "Assay development guidelines for image-based high content screening, high content analysis and high content imaging." Assay guidance manual [Internet] (2014).
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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!