Peroxisomes Imaging Services

Peroxisomes Imaging Services

Peroxisome is a small Membrane-enclosed organelle, which is related to various metabolic reactions of eukaryotic cells. The composition of peroxisomes largely depends on the cell type, tissue, and host organism. In human cells, peroxisomes are involved in the production of fatty acids β- Oxidation, detoxification of reactive oxygen species and biosynthesis of different lipids. Due to the important role of peroxisome in human metabolism, its functional defects can lead to many serious diseases. Therefore, visual analysis of the composition and function of peroxisome is very important for the control and treatment of peroxisome-related diseases.

Peroxisomal protein import processFigure 1. Peroxisomal protein import process (Galiani S, et al. 2016).

Peroxisomes Imaging Analysis

Membrane-related events during peroxisome protein introduction play an important role in peroxisome function.  At present, researchers can observe the morphological diversity of peroxisomes through the STED microscope, but they cannot individually visualize or qualitatively describe the heterogeneity of the spatial organization around peroxisomes during protein introduction. In order to overcome this difficulty, CD BioSciences uses quantitative imaging and analysis to describe the different characteristics of peroxisomes and accurately characterize peroxisomes and their components.

Peroxisomes Imaging Analysis Workflow

The following are the specific experimental steps of peroxisomes imaging analysis:

Cell culture

Cell culture

Human fibroblasts are maintained in a medium composed of DMEM and cultured at 37 °C and 5% CO2. This medium contains glucose, sodium pyruvate, 10% fetal bovine serum, etc.

Step 1
Cell transfection

Cell transfection

The cells were grown and transfected on the coverslip, and then the cells were fixed and permeabilized with PBS solution, after which the samples were incubated with the primary antibody in the blocking buffer for 1 h at room temperature.

Step 2


After several washing steps, the glass slide containing the sample was imaged under a STED microscope with various parameters set to observe the morphology of peroxisomes.

Step 3
Image analysis

Image analysis

The original image is processed to be corrected for morphological analysis, correlation analysis, coefficient calculation and zoning analysis.

Step 4


Image of peroxisome matrix and membrane protein
Spatial distribution of peroxisome regions
The compartmentalized spatial distribution of proteins on the peroxisome membrane
Intensity correlation coefficient
Intensity frequency histogram
Provide other data according to your needs

Our Advantages

  • Ability to display the position of multiple proteins in super-resolution mode
  • Use high spatial resolution STED microscope
  • Accurate characterization of peroxisomes and their components
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of peroxisomes imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with high-quality services that meet your needs.

  1. Galiani S, Waithe D, Reglinski K, et al. Super-resolution microscopy reveals compartmentalization of peroxisomal membrane proteins[J]. Journal of Biological Chemistry, 2016, 291(33): 16948-16962.
  2. Soliman K, Göttfert F, Rosewich H, et al. Super-resolution imaging reveals the sub-diffraction phenotype of Zellweger Syndrome ghosts and wild-type peroxisomes[J]. Scientific reports, 2018, 8(1): 1-13.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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