Glucose is a monosaccharide, which is an important energy source for almost all cells. Glucose metabolism includes glycolysis and gluconeogenesis. Glycolysis converts glucose into pyruvate or lactic acid, while gluconeogenesis converts simple non-sugar precursors (lactic acid, glycerol, glycogenic amino acids, etc.) into glucose. In addition to being related to energy metabolism, glucose metabolism also plays an important role in building block biosynthesis. Disorders of glucose metabolism are related to many diseases, such as cancer. Therefore, imaging analysis of the pathway of glucose metabolism in cells is very important for the diagnosis and treatment of diseases.

Figure 1. Gluconeogenesis GNG metabolic pathway for glucose generation outline diagram.

Glucose Metabolism Imaging Analysis

Traditionally, glucose metabolism in cell populations and tissues has been studied by tracking the uptake of radioactive metabolites by autoradiography or fluorodeoxyglucose (FDG) - PET scanning. However, radiolabeled glucose analogues will not be processed by hexokinase and are cytotoxic. In addition, the temporal and spatial resolution of isotope technology is low, which cannot measure the glucose metabolism of single cells, and cannot perform high-throughput analysis. With the continuous development of imaging technology, microscope imaging has become a powerful tool for high-resolution analysis of glucose metabolism in living cells.

CD BioSciences is a biotechnology company, which has been committed to the research and development of fluorescent probes and live cell imaging technology for many years. We can provide you with fluorescent probes for glucose metabolism analysis, and then monitor cell redox status and glucose flux changes in real time through high-content imaging.

Glucose Metabolism Imaging Analysis Workflow

The following are the specific experimental steps of glucose metabolism imaging analysis:

Delivery

Our Advantages

• Real-time monitoring of glucose metabolism at the single-cell level
• High-throughput imaging analysis
• High signal-to-noise ratio, low cytotoxicity
• Experienced scientists provide experimental consultation
• Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of glucose metabolism imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with high-quality services that meet your needs.

1. Maric, Tamara, et al. "Bioluminescent-based imaging and quantification of glucose uptake in vivo." Nature methods 16.6 (2019): 526-532.
2. Bittner, Carla X., et al. "High resolution measurement of the glycolytic rate." Frontiers in neuroenergetics 2 (2010): 26.
3. Kohnhorst, Casey L., et al. "Identification of a multienzyme complex for glucose metabolism in living cells." Journal of Biological Chemistry 292.22 (2017): 9191-9203.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!