Direct Multiplex Imaging of Bacterial Infections

Direct Multiplex Imaging of Bacterial Infections

Bacterial infection is a common clinical problem in acute and chronic wounds. Treatment of bacterial infections can only be done after an accurate diagnosis. Currently, the time-consuming bacterial culture may lead to unrecognized bacterial presence, delaying diagnosis. Therefore, it is very important to develop a rapid and accurate method for diagnosing bacterial infection. The combination of direct multiplex imaging of bacteria and Gram staining enables rapid and accurate diagnosis of bacterial infections.

Routine Gram stain shows identification of Gram negative (a P. aeruginosa) and Gram positive (b S. aureus) bacteria in both large aggregates (dotted lines) and individual bacteria within the biofilmFigure 1. Routine Gram stain shows identification of Gram negative (a P. aeruginosa) and Gram positive (b S. aureus) bacteria in both large aggregates (dotted lines) and individual bacteria within the biofilm (Becerra S C, et al. 2016).

Direct Multiplex Imaging Analysis of Bacterial Infections

Gram stain is a test that checks for bacteria at the site of infection. DMI can rapidly, specifically and reproducibly identify a variety of pathogenic microorganisms directly from samples, providing necessary information for timely and decisive treatment of diseases.

CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can combine Gram stain and DMI results to provide important information for the treatment of diseases caused by bacterial infections. Compared to other molecular diagnostic methods, our approach can reduce unnecessary concomitant treatment costs and save you time.

Detectable Bacterial Pathogens

Gram negative bacteria Gram positive bacteria
Acinetobacter spp. Staphylococcus spp.
Enterobacteriaceae Staphylococcus aureus
Escherichia coli Streptococcus spp.
Haemophilus influenzae Streptococcus pneumoniae
Klebsiella pneumoniae Enterococcus faecalis
Pseudomonas aeruginosa  
Serratia marcescens 
Stenotrophomonas maltophilia  
Proteus mirabilis  

The Workflow of Direct Multiplex Imaging Analysis of Bacterial Infections

The following are the specific steps of direct multiplex imaging analysis of bacterial infections

Sample preparation

Sample preparation

Step 1
Staining

Staining

Step 2
Imaging

Imaging

Step 3
Data analysis

Data analysis

Step 4

Delivery

High-quality sample images
Pathogen identification results
Other data that you need

Our Advantages

  • Specificity and sensitivity
  • Intact preserved cell morphology
  • Allows phenotyping and viability assessment
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacterial infections imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

Reference
  1. Becerra S C, Roy D C, Sanchez C J, et al. An optimized staining technique for the detection of Gram positive and Gram negative bacteria within tissue[J]. BMC Research Notes, 2016, 9(1).

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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