Mycoplasma Imaging Detection

Mycoplasma Imaging Detection

Mycoplasma is a kind of microorganism without cell wall, which shows resistance to many kinds of antibiotics. It is a widespread source of pollution in various cell culture systems and can cause a variety of effects on the cultured target cells, including metabolic changes, slowed proliferation, and chromosomal aberrations. Therefore, the visual monitoring of mycoplasma is very important to ensure that the cell line is not contaminated.

Bacteria mycoplasma genitalium.Figure 1. Bacteria mycoplasma genitalium.

Mycoplasma Imaging Detection

Cell culture is an essential part of the biopharmaceutical process. Early detection of mycoplasma contamination can reduce losses. The three most commonly used mycoplasma detection methods are: mycoplasma culture method, DNA staining method, and polymerase chain reaction method. CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We use DAPI counterstain to stain the mycoplasma nucleus, and then observe with a fluorescence microscope to detect mycoplasma contamination.

Mycoplasma Imaging Detection Workflow

The following are the specific steps of mycoplasma imaging detection:

Bacterial culture

Bacterial culture

Step 1
Transfer bacteria to 96-well plate

Transfer bacteria to 96-well plate

Step 2
Set parameters and time-lapse imaging

Set parameters and time-lapse imaging

Step 3
Image processing and data analysis

Image processing and data analysis

Step 4


Original images of mycoplasma, detection results and prediction results of Mycoplasma
Stained DNA image
Mycoplasma positive cell count
Provide other data according to your needs

Our Advantages

  • Reduce test time and improve the accuracy of results
  • Visualization of low mycoplasma concentration
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of mycoplasma imaging detection is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.

  1. Iseoka, Hiroko, et al. "Rapid and sensitive mycoplasma detection system using image-based deep learning." Journal of Artificial Organs (2021): 1-9.
  2. Wehbe, Katia, Marzia Vezzalini, and Gianfelice Cinque. "Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy." Analytical and bioanalytical chemistry 410.12 (2018): 3003-3016.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

Online Inquiry