DNA Editing Imaging Analysis
DNA editing is a highly specific modification of the DNA sequence of an organism. CRISPR is a powerful gene editing tool, which is widely used to correct genetic defects, treat and prevent disease transmission. It allows researchers to easily remove existing DNA base pairs and insert replacement base pairs to design DNA fragments with specific sequences. Therefore, it is very important to visualize DNA editing at the base pair level.
Figure 1. Vector illustration of the new science technique CRISPR-Cas (clustered regularly interspaced short palindromic repeats).
DNA Editing Imaging Analysis
Sequencing is a tool to identify and characterize edited DNA fragments, but the dynamic process of DNA editing cannot be monitored. In recent years, imaging technologies such as fluorescence in situ hybridization and fluorescent labeling have made real-time imaging of CRISPR-mediated DNA editing possible. However, traditional fluorescence in situ hybridization requires DNA denaturation, and the in vitro assembly of fluorescently labeled dCas9 and sgRNA only detects genomic sites in a fixed sample and cannot be tracked in real time.
CD BioSciences has been committed to the research and development of fluorescent probes and imaging technology for many years. In order to understand the mechanism of CRISPR/Cas9 and its off-target binding to DNA, visually study its binding position and dynamics at the base pair level in real time. We will provide you with a fluorescent probe that can be assembled with dCas9 to label the genome sequence in living cells to study the DNA editing process in real time.
DNA Editing Imaging Analysis Workflow
The following are the specific steps of DNA editing imaging analysis:
Fill out the online form to submit your DNA editing project.
Send us the DNA to be edited or the cells containing the target DNA.
We use fluorescent probes to label the genome sequence in living cells to study the DNA editing process in real time.
After editing, we will send the sample and experimental data to you.
Real-time observation of DNA structure
Real-time location of genome location
The position and intensity of the dCas9-DNA interaction
Provide other data according to your needs
- Ability to track the dynamics of DNA editing events
- Minimize background noise
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has a professional team and advanced imaging equipment. The entire process of DNA editing imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.
- Wang, Haifeng, et al. "CRISPR-mediated live imaging of genome editing and transcription." Science 365.6459 (2019): 1301-1305.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!