Lipid Droplets Imaging Analysis
As a structural component of biofilm, biosynthetic precursor, signal converter and energy storage, lipids play a key role in cell physiology. Mammalian cells store excess lipid molecules in specialized intracellular organelles, called lipid droplets (LD), also known as adiposomes. Lipid droplets participate in maintaining lipid homeostasis through lipid synthesis, metabolism and transport. Based on the control of these important cellular functions, LDs is closely related to human diseases such as dyslipidemia, diabetes, obesity and fatty liver disease. Therefore, fluorescence imaging has attracted great attention as an important tool to study the formation, growth, fusion and contraction mechanism of LD.
Figure 1. Fluorescence imaging of brown adipocytes stained with Lipi-probes (Ferrara M A, et al. 2019).
Lipid Droplets Imaging Analysis
LD-specific fluorescent dyes are a powerful tool for monitoring the morphological and dynamic relationship between LD and other organelles. Since LDs are not homogeneous in content, there is no single marker available to measure the entire population of LDs. Currently, the method of monitoring the morphological and dynamic interactions between LD and other organelles is to stain the cells with fluorescent dyes, and then perform multi-color imaging through a fluorescence microscope. These fluorescent dyes have a strong affinity for the neutral lipids of LD, but they will non-specifically bind to lipophilic sites other than LD in the cell to produce a fluorescent background. Therefore, it is necessary to develop highly specific and retainable LD fluorescent probes.
CD BioSciences has an R&D team with rich experience and expertise in the imaging field. We can develop LD high specific fluorescent probes with very low fluorescence background for fluorescence imaging and kinetic monitoring of LDS in living cells. They can not only be used to monitor several different organelles at the same time, but also can be used for the "time lag staining" of LDs in a single cell, which is helpful to study the mechanism of cell events related to LDS.
Lipid Droplets Imaging Analysis Workflow
The following are the specific experimental steps of lipid droplets imaging analysis:
Step 1: Cell culture
The pre-adipocytes are inoculated into a suitable medium and differentiated into mature adipocytes.
Step 2: Immunofluorescence
Lipid droplets were stained with a neutral lipid stain and kept at room temperature for 30 minutes, then wash the cells thoroughly with PBS.
Step 3: Imaging
Step 4: Image analysis
Lipid droplet formation and intercellular metastasis of LDS were analyzed by image analysis.
Location, distribution and biophysical characteristics of LDs
LDs intracellular dynamics
Other relevant data
- High-sensitivity optical system to capture high-quality pictures
- Very low background fluorescence level
- Low toxicity of LD-specific probes
- Long-term monitoring of LD dynamics in single cells
- Experienced scientists provide experimental consultation
- Reasonable price and short turnaround time
CD BioSciences has a professional team and advanced imaging equipment. The entire process of lipid droplets imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will provide you with personalized lipid droplets imaging analysis services according to your needs.
- Ferrara M A, Filograna A, Ranjan R, et al. Three-dimensional label-free imaging throughout adipocyte differentiation by stimulated Raman microscopy[J]. Plos one, 2019, 14(5): e0216811.
- Tatenaka Y, Kato H, Ishiyama M, et al. Monitoring lipid droplet dynamics in living cells by using fluorescent probes[J]. Biochemistry, 2019, 58(6): 499-503.
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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!