Acid-Fast Bacteria Imaging Services

Acid-Fast Bacteria Imaging Services

Acid-fast bacteria (AFB) are a group of bacteria with acid-fast properties. The outer membrane of its cell wall contains 40% to 60% mycobacterial acid. Acid resistance is a physical property, which makes it impossible to decolorize bacteria with acid alcohol bleaching agents after they are stained. Therefore, it is very important to select appropriate fluorescent dyes for imaging analysis of acid-resistant bacteria.

Mycobacterium positive (small red rod) in acid fast stain sputum.Figure 1. Mycobacterium positive (small red rod) in acid fast stain sputum.

Acid-Fast Bacteria Imaging Analysis

Many human diseases are caused by acid-fast bacteria, among which tuberculosis is one of the most common diseases. Acid-fast staining and microscopy are one of the methods to visually analyze acid-fast bacteria, which can help researchers diagnose and treat diseases caused by acid-fast bacteria. CD BioSciences has been committed to the development of fluorescent dyes and imaging technology for many years. We can develop an automatic imaging and analysis scheme for acid-fast bacteria screening based on acid fast staining and digital wide field microscope.

Acid-Fast Bacteria Imaging Analysis Workflow

The following are the specific steps of acid-fast bacteria imaging analysis:

Sample preparation

Sample preparation

Step 1
Sample staining

Sample staining

Step 2
Microscope imaging

Microscope imaging

Step 3
Image analysis

Image analysis

Step 4


Original image of acid-fast bacteria
Total number of acid-fast bacteria
Provide other data according to your needs

Scatter plot of montage analysis results
The final score of the smear montage image

Our Advantages

  • High resolution imaging
  • Improve the reliability and accuracy of acid-fast bacteria counts
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of acid-fast bacteria imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

  1. Bayot M L, Mirza T M, Sharma S. Acid Fast Bacteria[J]. 2019.
  2. Law Y N, Jian H, Lo N W S, et al. Low cost automated whole smear microscopy screening system for detection of acid fast bacilli[J]. Plos one, 2018, 13(1): e0190988.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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