Chemotaxis Cell Migration & Invasion Imaging Services
Cell migration and invasion are vital in development, immune response, cancer metastasis, inflammation, and wound healing. In healthy conditions, migration is regulated, but in diseases like cancer, it can become uncontrolled, leading to metastasis. Measuring cell chemotaxis is crucial for understanding disease progression. However, current imaging assays can't observe real-time cell morphology, and lack comprehensive quantitative analyses of cell movement kinetics.
Fig. 1 Chemotaxis and chemotactic gradients (Germán Reig, et al. 2014).
Chemotaxis Cell Migration & Invasion Imaging Services
CD BioSciences provides a method for detecting chemotaxis cell migration and invasion based on 96-well/24-well plate imaging of living cells. Unlike traditional assays that provide single time-point measurements, our method enables continuous visualization and reproducible analysis of live cell motility. Our service can help clients to obtain the following information:
- Obtain full time-scale migration profiles of chemotactic cells for a comprehensive view of cell behavior.
- Used to study the effects of treatment on cell migration and invasion, screen for therapeutic effects that inhibit invasion, and gain insight into cell migration pathways across the endothelium.
- Accelerate the research process and the development of therapeutic strategies targeting cell migration and invasion.
Our Cell Migration Imaging Services
Chemotaxis cell migration assay services
Haptotaxis cell migration assay services
Chemotactic T cell migration induction services
Chemotactic T cell migration inhibition services
Chemotactic primary T cell migration services
Endothelial cell migration assay services
Leukocyte transendothelial migration services
Tumor cell transendothelial migration services
Human neutrophil chemotaxis induction services
Human neutrophil chemotaxis inhibition services
Murine neutrophil chemotaxis induction services
Our Cell Invasion Imaging Services
Endothelial cell invasion assay services
Cancer cell invasion imaging service
Immune cell invasion Imaging services
Our Technology
- Boyden Chamber Assay (Transwell Migration Assay)
It is a widely used technique for cell migration. The method involves placing cells in a chamber with a porous membrane and allowing them to migrate towards chemoattractants. The migrating cells are then stained and counted.
- Microfluidic Migration Assay
Through establishing a stable concentration gradient at specific time intervals, images of the observation area can be acquired for real-time monitoring and quantitative measurement of cell migration, thus overcoming the limitations of traditional detection methods.
- In Vitro Scratch Assay
Produce multiple scratch wounds to study cell migration in a higher throughput manner and image migrating cells in vivo.
Chemotaxis Cell Migration & Invasion Imaging Workflow
Cell seeding
Cells are seeded in the specific 96-well plate.
Add chemoattractant
Add chemoattractants or inserts to the lower reservoir plate.
Live cell imaging
Visualize and dynamically monitor chemotaxis cell migration in real time by live cell imaging.
Image Acquisition
Acquire and process whole well images for data analysis and quantification.
Our Advantages
- Allows real-time quantification and visualization of cell migration or invasion for each cell.
- Enables highly reproducible kinetic assays.
- Enable acquire cell morphology of each cell in the well.
CD BioSciences has a professional team and advanced imaging equipment. The whole process of chemotaxis cell migration and invasion imaging service is operated by experienced technicians to ensure the accuracy of the experiment. We can also customize chemotaxis cell migration and invasion imaging services for you. If you have any needs, please feel free to contact us.
- Germán Reig, Eduardo Pulgar, Miguel L. Concha. Cell migration: from tissue culture to embryos. Development 15 May, 2014, 141 (10): 1999–2013.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!