Neuronal Activity Imaging Services
Human induced pluripotent stem cell (hiPSC)-derived neurons have become a powerful tool for studying basic developmental mechanisms and complex diseases of the nervous system. hiPSC-derived cell models are widely used in fields such as molecular and developmental neuroscience, drug development, and neurotoxicology. These models can help researchers gain insights into the functions associated with neuronal networks and disease states. Using advanced imaging techniques, such as live-cell imaging, calcium imaging, or sensitive dyes, we can visualize and track the activity of individual neurons activity or neuronal networks in real time, revealing the dynamic processes of neuronal function and dysfunction. In addition, neuronal activity imaging can identify therapeutic targets for neurological diseases and assess the efficacy of potential drug candidates.
Neuronal Activity Imaging Services
CD BioSciences' neuronal activity imaging services can help our clients overcome the limitations of temporal and spatial resolution imposed by traditional imaging techniques such as electrophysiologic recording. We will utilize calcium indicators and live cell imaging techniques to obtain complex neuronal activity measurements, enabling clients to gain insight into the function of neuronal cell models and explore the complexity of the nervous system. Our neuronal activity imaging services include, but are not limited to the following:
Long-term study of activity
Enable make long-term observations of neuronal activity, identify changes in activity patterns over weeks or months, and quantify longitudinal changes in activity to characterize neuronal cell models under physiologically relevant conditions.
Compare neuronal activity across genotypes
Our service can compare the network activity of neuronal cultures from different genotypes, enabling our customers to understand the effect of different genotypes on neuronal activity.
Multiple culture condition testing
We will test the activity of multiple neuronal lines under multiple culture conditions to assess the impact of co-culture between different cell types.
Analysis of calcium transients in stem cell-derived neurons
- Longitudinal analysis of calcium transients in stem cell-derived neurons.
- Provides highly repeatable measurements of burst rate, burst duration, and network formation.
- Observe cell morphology to exclude outliers and ensure accurate analysis.
Detailed activity characterization
Label neurons with calcium indicators and analyze calcium dynamics in each neuron's activity using live-cell imaging equipment, providing a comprehensive characterization of neuronal activity.
Neuronal Activity Imaging Services Workflow
Step 1
Experimental protocol confirmation
According to the research requirements, confirm the experimental protocol, experimental conditions, sample types, etc.
Step 2
Calcium Indicator Labeling
Choose the appropriate calcium indicator to label the neuronal samples according to the needs of the experimental protocol, commonly used calcium indicators include Fura-2, Fluo-4 and GCaMP.
Step 3
Live cell imaging
Neuronal activity is triggered by stimulation of the neuronal sample, the labeled neuronal sample is imaged using the live cell imaging device, and signal changes in the neuronal sample are recorded, (the length and interval of the imaging depends on the research purpose).
Step 4
Process and analyze data
Analyze and process imaging data using specialized software and algorithms. Calculate changes in calcium ion concentration, detect the location of active synapses or neurons, and analyze neuronal activity.
Our Advantages
- High spatial and temporal resolution rapidly captures dynamic changes in neural activity.
- Calcium indicators are used to achieve imaging by adding fluorescent dyes or genetically encoded indicators to living cells without traumatic manipulation of cells or animals, better preserving the physiological state of cells and tissues.
- Real-time imaging can be performed to continuously record changes in neural activity, observe and analyze transient neuronal activity and study interactions between neurons.
- Allows continuous recording of neural activity over a long period of time, helping to study changes in neural activity such as chronic diseases.
CD BioSciences has a professional team and state-of-the-art imaging equipment to provide you with neuronal activity imaging services. If you have any needs, please feel free to contact us. We can also customize neuronal activity imaging services for you according to specific needs.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!