Bacterial Cell Wall Synthesis Imaging Services

Bacterial Cell Wall Synthesis Imaging Services

Cell wall is an important part of bacterial structure, and its main component is peptidoglycan (PG). In addition to maintaining the shape of bacteria, the cell wall can also act as a scaffold for the attachment of other cell components and help bacteria counteract the osmotic pressure in cells. Therefore, PG is an essential and unique component of bacteria, and the complex mechanism of dynamic synthesis and degradation of peptidoglycan has also become the focus of research in this field. The development of microscope technology provides an effective method for the study of peptidoglycan biosynthesis, so that researchers can visually analyze the kinetics and mechanism of peptidoglycan biosynthesis.

Structure of the bacterial cell wall in different types of bacteriaFigure 1. Structure of the bacterial cell wall in different types of bacteria (Radkov A D, et al. 2018).

Bacterial Cell Wall Synthesis Imaging Analysis

Bacteria can accurately adjust their PG structure according to the changes of environment, so the structure of PG is highly dynamic. Traditionally, researchers have used electron microscopy, fluorescence microscopy and atomic force microscopy to study how bacteria coordinate PG synthesis and its dynamic structure. However, these microscopes are limited by resolution, which makes detailed studies of PG structure and protein behavior very difficult. With the advent of super-resolution microscopy, we have been able to visually track the synthesis and dynamic structure of live bacterial PG at the single-molecule level.

CD BioSciences has been committed to the development of fluorescent probes and imaging technology for many years. We can use super-resolution microscopy to visually analyze the biosynthesis of PG and the molecular interactions in the process.

Bacterial Cell Wall Synthesis Imaging Analysis Workflow

The following are the specific steps of bacteria cell wall synthesis imaging analysis:

Synthetic Fluorescent Dyes

Synthetic Fluorescent Dyes

Step 1
Strain construction

Strain construction

Step 2
Single molecule imaging and tracking

Single molecule imaging and tracking

Step 3
Single-cell imaging of growth

Single-cell imaging of growth

Step 4
Data processing and analysis

Data processing and analysis

Step 5

Delivery

Molecular probes for studying the synthetic mechanism of peptidoglycan
High-resolution images collected during peptidoglycan synthesis
Kinetics of peptidoglycan synthesis
Peptidoglycan-enzyme interaction
Peptidoglycan metabolism kinetics
Other data that you need

Our Advantages

  • High resolution and signal-to-noise ratio
  • Allows live bacteria imaging and time-lapse monitoring
  • Study the biosynthesis of PG at the molecular level
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of bacteria cell wall synthesis imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

References
  1. Radkov A D, Hsu Y P, Booher G, et al. Imaging bacterial cell wall biosynthesis[J]. Annual review of biochemistry, 2018, 87: 991-1014.
  2. Lee T K, Meng K, Shi H, et al. Single-molecule imaging reveals modulation of cell wall synthesis dynamics in live bacterial cells[J]. Nature communications, 2016, 7(1): 1-9.
  3. Hernández S B, Cava F. New approaches and techniques for bacterial cell wall analysis[J]. Current opinion in microbiology, 2021, 60: 88-95.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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