Imaging-based Wound Healing Assay

Imaging-based Wound Healing Assay

The wound healing test, also known as the scratch test, involves forming a uniform wound or scratch on a cell monolayer and observing the migration of cells around the edge of the wound to a cell-free space through time-lapse imaging. It is a simple and cost-effective method for measuring mass cell migration in vitro. Collective cell migration is essential for various biological processes including embryonic development, immune response, and cancer metastasis. Therefore, the quantitative analysis of the wound healing process is very important.

Wound healing assays study cell migration and cell-cell interactions in vitroFigure 1. Wound healing assays study cell migration and cell-cell interactions in vitro (Kauanova S, et al. 2021).

Imaging-based Wound Healing Assay

CD BioSciences can provide you with image-based wound healing assay services. We use automatic microscope imaging for wound healing testing. Such a system allows you to take images regularly (for example, every 30 minutes or 1 hour) to closely monitor cell migration and quickly generate accurate and repeatable analysis results.

Imaging-based Wound Healing Assay Workflow

Wound healing assay is one of the most commonly used testing methods in the field of cell migration, which is used to study cell migration and cell-cell interactions in vitro. The main steps include creating a wound in a cell monolayer, periodically capturing images during cell migration until the wound is closed, and then analyzing the image sequence to evaluate cell migration. The following is the workflow of imaging-based assay of wound healing.

Step 1

Cell seeding and gap formation

Step 2

Acquisition of microscopy images

Step 3

Quantitative image analysis

Step 4

Data analysis and evaluation


Percentage of wound closure
Cell migration
Wound closure rate
Other relevant data

Our Advantages

  • Automatic high-quality image acquisition
  • Quickly generate accurate and reliable image analysis results
  • High-throughput detection, simple and cost-effective
  • Reducing data discrepancies caused by operations
  • Experienced scientists provide experimental consultation

CD BioSciences has a professional team and advanced imaging equipment. The entire process of wound healing assay is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us, we can provide you with high-quality image-based wound healing assay services.

  1. Kauanova S, Urazbayev A, Vorobjev I. The Frequent Sampling of Wound Scratch Assay Reveals the "Opportunity" Window for Quantitative Evaluation of Cell Motility-Impeding Drugs[J]. Frontiers in cell and developmental biology, 2021, 9: 391.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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