Steatosis and Phospholipidosis Imaging Analysis

Steatosis and Phospholipidosis Imaging Analysis

Steatosis refers to the accumulation of triglycerides (neutral fat) in the cytoplasm, which is often caused by nutritional disorders, infection, poisoning, and hypoxia. It usually occurs in tissues with strong metabolism and high oxygen consumption, such as hepatocytes, myocardial fibers, and renal tubular epithelium. Severe steatosis cells can develop into necrosis. Drug-induced steatosis is characterized by excess triglyceride accumulation in the form of lipid droplets in liver cells. Under the electron microscope, the fat in the cytoplasm of the cell appears as adipose bodies, which then fuse into lipid droplets.

Electron microscopic images of steatosis and phospholipidosis.Figure 1. Electron microscopic images of steatosis and phospholipidosis. (Sahini N, et al. 2014).

Steatosis and Phospholipidosis Imaging Analysis

CD BioSciences can provide you with professional steatosis and phospholipidosis imaging analysis services. The lipid droplet-selective small-molecule fluorescent probes we developed can be used for one-photon and two-photon microscopes utilizing live cells or fixed cells. It provides a feasible method for screening potential steatosis and/or phospholipid degeneration drugs.

Analysis method High content imaging
Cell types available HepaRG (with and without non-parenchymal cells), HepG2, primary hepatocytes (with and without non-parenchymal cells)
Time point(s) 48 h, 7 day, or 14 day, other time points available on request
Sample requirements DMSO solution, or equivalent amount in solid compound

Steatosis and Phospholipidosis Imaging Analysis Workflow

Our steatosis and phospholipidosis imaging analysis combined high-content screening with 3D cell model to provide a medium-throughput method to screen test compounds to determine their potential to induce steatosis. The analysis steps are as follows.

Step 1

Cultivate the 3D hepatocyte model on the U-bottom plate to a diameter of about 200μm.

Step 2

Treatment of test compound and reference substance.

Step 3

Fix the 3D model and treat it for fluorescent labeling.

Step 4

The 3D model uses lipid stain to label lipids and phospholipids, and DAPI to label the nucleus.

Step 5

Perform high-content imaging and image analysis on well plates to quantify lipids and phospholipids in cell models.


Dose-response curves
AC50 values and minimum toxic concentration

Our Advantages

  • A variety of fluorescent probes can be used to evaluate the lipid droplets in liver cells
  • Comprehensive data can be provided
  • Cost-effective and customized services
  • Accurate results can be obtained in a short time
  • Experienced scientists provide experimental consultation

CD BioSciences has a professional team and advanced imaging equipment. The entire process of steatosis and phospholipidosis imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you are looking for related services, please feel free to contact us.

  1. Sahini N, Selvaraj S, Borlak J, et al. Whole genome transcript profiling of drug induced steatosis in rats reveals a gene signature predictive of outcome [J]. PLoS One, 2014, 9(12): p.e114085.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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