Kidney Toxicity Imaging Analysis
The kidneys play an important role in maintaining the homeostasis of the body's internal environment, including regulating water, electrolyte, nitrogen, and acid-base balance. They also control red blood cell production and blood pressure. Impaired renal function is common in clinical practice and is usually related to the use of drugs. Therefore, early prediction of drug nephrotoxicity is very important for drug development and market entry.
Figure 1. Human kidney anatomy. External view (a), internal view (b), and its functional unit nephron (c) (Faria, João, et al, 2019).
Kidney Toxicity Imaging Analysis
An in vitro model was developed to reliably predict the nephrotoxicity of potential drugs. The two-dimensional culture of primary or conditionally immortalized renal epithelial cells represents the gold standard of traditional in vitro model for nephrotoxicity evaluation. However, proximal tubular epithelial cells cultured with this method exhibit poor apical-basal polarization and low levels of key protein transporters, which hinder their utility as a tool for predicting drug nephrotoxicity.
In order to bypass the limitations of 2D culture, CD BioSciences uses the advanced in vitro culture system that has been developed, including organoids and renal micro physiological system that can reproduce the characteristics of fluids in the body to simulate in vivo tissues, so as to provide a reliable tool for nephrotoxicity assessment in the early stage of drug development.
| Analysis method | High content imaging |
| Cell type | Renal proximal tubule epithelial cells |
| Toxicity markers | DNA structure, mitochondrial mass, mitochondrial membrane potential, phospholipidosis, glutathione content, cellular ATP |
| Sample requirements | 150 µL of a stock solution or equivalent amount in solid compound |
Kidney Toxicity Imaging Analysis Workflow
We use the high-content imaging analysis system to provide you with drug nephrotoxicity analysis services for human renal proximal tubule epithelial cells. The detailed steps are as follows:
Step 1
Cultivate renal proximal tubule epithelial cells to create 3D models.
Step 2
Treat 3D models with test compound.
Step 3
Tissue removal and performs high-content imaging.
Step 4
Analyze the experimental data and evaluate nephrotoxicity of the drug.
Delivery
Minimum effective concentration
AC50 values with dose-response curves for each measured parameter
Our Advantages
- Allows cell-level analysis while imaging occurs
- Provide samples with better light transmittance and excellent image quality
- Reduce the error caused by human operation
- Reduced workload and cost
CD BioSciences has a professional team and advanced imaging equipment. The entire process of kidney toxicity imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us. We will design a personalized analysis plan for you according to your project, and provide you with accurate experimental data and analysis reports.
- Faria, João, et al. "Kidney-based in vitro models for drug-induced toxicity testing." Archives of toxicology 93.12 (2019): 3397-3418.
- Angelotti, Maria Lucia, et al. "Imaging the kidney: from light to super-resolution microscopy." Nephrology Dialysis Transplantation 36.1 (2021): 19-28.
*If your organization requires the signing of a confidentiality agreement, please contact us by email.
Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!