GABAA Receptors Imaging Analysis

GABAA Receptors Imaging Analysis

GABAA receptors are the main inhibitory neurotransmitter receptors in the central nervous system, which can inhibit nerve excitability by mediating the influx of extracellular chloride ions. The localization and distribution of GABAA receptors on the nerve cell membrane plays an important role in regulating the generation and transmission of nerve signals. A large number of studies have shown that the dysregulation of GABAA receptors in the cell membrane distribution level is closely related to the occurrence of neurological diseases. In addition, GABAA receptors are also important drug targets for central nervous system diseases. Therefore, imaging analysis of the structure and transport of GABAA receptors is of great importance.

Overall structure of the GABAA receptor-Fab complexFigure 1. Overall structure of the GABAA receptor-Fab complex (Zhu S, et al. 2018).

GABAA Receptors Imaging Analysis

For a long time, X-ray diffraction has been regarded as the gold standard of structural biology. It requires protein crystallization and determines the protein structure according to the X-ray diffraction pattern. However, the three-dimensional structure of GABAA receptor is difficult to be analyzed by X-ray diffraction crystal analysis.

CD BioSciences has been committed to the development of imaging technology and fluorescent dye for many years. We can use confocal fluorescence microscopy and high-resolution electron microscopy to characterize the three-dimensional structure of GABAA receptors in cells. In addition, we can track the movement of individual GABAA receptors within living cells via genetically encoded fluorophores.

GABAA Receptors Imaging Analysis Workflow

The following are the specific steps of GABAA receptors imaging analysis

Cell culture

Cell culture

Step 1
Sample preparation

Sample preparation

Step 2


Step 3
Data analysis

Data analysis

Step 4


High-resolution microscope images of GABAA receptors
High-resolution structure of the GABAA receptors
Location and transport of intracellular GABAA receptors
Other data that you need

Our Advantages

  • Studying the spatial distribution of cell surface GABAA receptors at the nanoscale
  • Live cell imaging can visually analyze receptor trafficking
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of GABAA receptors imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

  1. Orlando M, Ravasenga T, Petrini E M, et al. Correlating fluorescence and high-resolution scanning electron microscopy (HRSEM) for the study of GABAA receptor clustering induced by inhibitory synaptic plasticity[J]. Scientific Reports, 2017, 7(1): 1-10.
  2. Zhu S, Noviello C M, Teng J, et al. Structure of a human synaptic GABAA receptor[J]. Nature, 2018, 559(7712): 67-72.
  3. Lorenz-Guertin J M, Wilcox M R, Zhang M, et al. A versatile optical tool for studying synaptic GABAA receptor trafficking[J]. Journal of Cell Science, 2017, 130(22): 3933-3945.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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