Epidermal Growth Factor Receptor Imaging Analysis

Epidermal Growth Factor Receptor Imaging Analysis

Epidermal growth factor receptor (EGFR) is a huge transmembrane glycoprotein with a molecular weight of about 180KDa and has ligand-induced tyrosine protein kinase activity. EGFR is involved in the regulation of various physiological processes such as cell proliferation and differentiation and apoptosis, and is overexpressed or mutated in various epithelial cancers, gliomas, and glioblastomas. Therefore, imaging analysis of EGFR is very important for the treatment of these types of cancers.

Molecular model of the EGFR dimerFigure 1. Molecular model of the EGFR dimer (Peckys, et al. 2013).

Epidermal Growth Factor Receptor Imaging Analysis

Currently, there are limited methods to directly visualize proteins and complexes with high spatial resolution in the complete cell membrane, which hinders researchers from understanding the spatial structure of EGFR on the cell surface. In recent years, with the development of single molecule fluorescence and single particle tracking technology, the structure and mechanism of EGFR dimer and oligomer have been widely studied, but the entire receptor population cannot be revealed in most cases.

CD BioSciences has been committed to the development of fluorescent probe and imaging technology for many years. We can use near-field scanning optical microscope (NSOM) to image EGFR in cells at the nano level and to study the distribution of EGFR and the structure of its aggregates.

Epidermal Growth Factor Receptor Imaging Analysis Workflow

The following are the specific steps of epidermal growth factor receptor imaging analysis

Cell culture

Cell culture

Step 1
Sample preparation

Sample preparation

Step 2
Imaging

Imaging

Step 3
Data analysis

Data analysis

Step 4

Delivery

EGFR localization images
Correlation of EGFR cluster area and intensity
EGFR square
EGFR histogram under different conditions
Other data that you need

Our Advantages

  • Fluorescence imaging with sub-diffractive resolution
  • Visual analysis of EGFR aggregation in cells
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has a professional team and advanced imaging equipment. The entire process of epidermal growth factor receptor imaging analysis is operated by experienced technicians to ensure the accuracy of the experiment. If you have any needs, please feel free to contact us.  

References
  1. Abulrob A, Lu Z, Baumann E, et al. Nanoscale imaging of epidermal growth factor receptor clustering: effects of inhibitors[J]. Journal of Biological Chemistry, 2010, 285(5): 3145-3156.
  2. Diagaradjane P, Orenstein-Cardona J M, Colón-Casasnovas N E, et al. Imaging epidermal growth factor receptor expression in vivo: pharmacokinetic and biodistribution characterization of a bioconjugated quantum dot nanoprobe[J]. Clinical Cancer Research, 2008, 14(3): 731-741.
  3. Peckys, Diana B., et al. Epidermal growth factor receptor subunit locations determined in hydrated cells with environmental scanning electron microscopy[J]. Scientific Reports, 2013,3(1): 1-8.

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Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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