Solvent-Based 3D Cell Culture Clearing Kit
This high lipid version tissue clearing kit is designed to support the CLARITY method of tissue clearing, offering a range of benefits for researchers. By enabling non-destructive tissue processing and facilitating 3D imaging, it empowers the examination of both normal and diseased soft tissues with exceptional precision. Specifically tailored for lipid clearing of soft tissues such as brain tissue, eye, and lymph nodes.
Information
Description:User-friendly solution for reversible and non-destructive tissue clearing and staining of organoids, 3D cell cultures and microtissues. Achieve exceptional results without shrinkage or damage. Compatible with fluorescent protein labeling, immunolabeling, small molecule staining, and all standard dyes and buffers.
Clearing Principle:Solvent-based tissue clearing
Kit Contents:3D cell culture clearing reagent
Tissue clearing antibody buffer
Tissue clearing blocking buffer
Tissue clearing penetration buffer
Tissue clearing washing buffer 10X
Tissue clearing antibody buffer
Tissue clearing blocking buffer
Tissue clearing penetration buffer
Tissue clearing washing buffer 10X
Form:Liquid
Storage:Store at RT
Technology:This method operates by following a sequence of steps: dehydration, delipidation, and refractive index matching of your sample.
Dehydration involves exposing the tissue to escalating concentrations of alcohol to remove moisture. Subsequently, the sample is immersed in a solution of organic solvent, which delipidates the tissue and envelops protein-based structures, rendering them transparent.
Dehydration involves exposing the tissue to escalating concentrations of alcohol to remove moisture. Subsequently, the sample is immersed in a solution of organic solvent, which delipidates the tissue and envelops protein-based structures, rendering them transparent.
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