3-Color IHC Kit for 1 Mouse/2 Rabbit Antibody on Rodent Tissue, Brown/Red/Black

3-Color IHC Kit for 1 Mouse/2 Rabbit Antibody on Rodent Tissue, Brown/Red/Black

3-Color IHC Kit for 1 Mouse/2 Rabbit Antibody on Rodent Tissue, Brown/Red/Black

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Catalog NOMCIS082


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Description
Triple staining employs both conventional and innovative techniques in immunohistostaining to unveil the presence of three separate antigens and their simultaneous expression within a single tissue. The 3-Color IF Kit is designed to use DAB(brown), Permanent Red (red), and DAB-Ni (Black) dye effectively stain 3 different antigens on rodent tissue or cell samples when paired with user-supplied 1 mouse and 2 rabbit primary antibodies.
Information
Kit Contents HRP-Polymer anti-Rabbit (RTU)
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rabbit (RTU)
Mouse Primer (RTU)
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Antibody Blocker (40x)
Mouse-Rabbit-Rabbit Blocker 1 (RTU)
Mouse-Rabbit-Rabbit Blocker 2 (RTU)
DAB-Ni Substrate (20x)
Hydrogen Peroxide (20X)
Nickel Solution (7x)
Mounting Medium (RTU)
Usage The kit provides polymer enzyme conjugates, including polymer-HRP anti-mouse IgG, polymer-HRP anti-rabbit IgG, and polymer-AP anti-rabbit IgG, along with three substrates/chromogens: DAB (brown), Permanent Red (red), and DAB-Ni (black). This kit is designed as a non-biotin system to avoid nonspecific binding caused by endogenous biotin.
Applications Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color DAB (brown),Permanentred (red),and DAB-Ni (black)
Specificity Mouse and Rabbit
Tissue Species Rodent
Storage 2-8℃
Note The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.

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