2-Color IHC Kit for Mouse/Rat Antibody on Rodent Tissue, Purple/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use BCIP/NBT (Purple) and AEC (Red) dye effectively stain 2 different antigens on mouse tissue or cell samples when paired with user-supplied mouse and rat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Rat (RTU)
BCIP/NBT (RTU)
Mouse-Rat Blocker 1 (RTU)
Mouse-Rat Blocker 2 (RTU)
Mouse Primer (RTU)
AEC Substrate (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
AP-Polymer anti-Rat (RTU)
BCIP/NBT (RTU)
Mouse-Rat Blocker 1 (RTU)
Mouse-Rat Blocker 2 (RTU)
Mouse Primer (RTU)
AEC Substrate (20x)
AEC Chromogen (20x)
Hydrogen Peroxide (20x)
Mounting Medium (RTU)
Usage
The kit contains two polymer enzyme conjugates: Mouse HRP (AEC) Polymer and Rat AP Polymer. These conjugates are accompanied by two distinct substrates/chromogens. The Mouse HRP Polymer uses AEC, producing a red color, while the Rat AP Polymer uses BCIP/NBT Red, resulting in a purple color.
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
BCIP/NBT (purple) and AEC (red)
Specificity
Mouse and Rat
Tissue Species
Mouse
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
A primer step is employed to enhance the specificity of antibody staining. This kit offers a simplified protocol that is faster and easier compared to a sequential procedure. It utilizes a non-biotin system to prevent non-specific binding of endogenous biotin.