2-Color IHC Kit for Goat/Rabbit Antibody on Human/Rodent Tissue, Brown/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use DAB (brown) and Permanent Red (red) dye effectively stain 2 different antigens on human and mouse tissue or cell samples when paired with user-supplied goat and rabbit primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Goat (RTU）
AP-Polymer anti-Rabbit (RTU）
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
AP-Polymer anti-Rabbit (RTU）
DAB Substrate (RTU)
DAB Chromogen (20x)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Mounting Medium (RTU)
Usage
The kit provides two polymer enzyme conjugates: HRP Polymer anti-Goat IgG and AP Polymer anti-Rabbit IgG. These conjugates are accompanied by two substrates/chromogens: DAB (brown color) and Permanent Red (red color).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
DAB (brown) and Permanentred (red)
Specificity
Goat and Rabbit
Tissue Species
Human and Rodent
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
If only the anti-goat antigen is present, HRP polymer will react with DAB (brown) chromogen, resulting in its presence. Conversely, if only the anti-rabbit antigen is present, AP polymer will react exclusively with GBI-Permanent Red (red) chromogen. If both rabbit and goat antigens are present, both DAB and GBI-Permanent Red chromogens will be present. The kit is a non-biotin system that eliminates the need for blocking steps to prevent non-specific binding of endogenous biotin.