2-Color IHC Kit for Goat/Mouse Antibody on Human Tissue, Geen/Red
In immunohistochemistry, the technique of double staining is widely employed to simultaneously detect and differentiate two distinct antigens within a single tissue sample. The 2-Color IF Kit is designed to use Emerald (Green) and Permanent Red (Red) dye effectively stain 2 different antigens on human tissue or cell samples when paired with user-supplied mouse and goat primary antibodies.
Information
Kit Contents
HRP-Polymer anti-Mouse (RTU)
AP-Polymer anti-Goat (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Double Stain Blocker
Mounting Medium (RTU)
AP-Polymer anti-Goat (RTU)
Permanent Red Substrate (RTU)
Permanent Red Activator (5x)
Permanent Red Chromogen (100x)
Emerald Chromogen (RTU)
Double Stain Blocker
Mounting Medium (RTU)
Usage
The kit provides two polymer enzyme conjugates: AP polymer anti-Goat IgG and HRP polymer anti-Mouse IgG. It includes two distinct substrates/chromogens: Emerald (Green) and Permanent Red (Red).
Applications
Paraffin tissue (verified), frozen specimen and freshly prepared monolayer cell smears.
Color
Emerald (green) and Permanentred (red)
Specificity
Goat and Mouse
Tissue Species
Human
Storage
2-8℃
Note
The outcome is significantly influenced by several factors, including fixation, tissue slide thickness, antigen retrieval, as well as the dilution and incubation time of the primary antibody. It is crucial for the investigator to carefully evaluate all of these variables and establish the ideal conditions in order to accurately interpret the results.
Principle
This simplified protocol offers convenience by sequentially applying the enzyme conjugates to the specimen. By utilizing this kit, researchers can visualize the co-localization of two proteins based on the resulting color change when the chromogens overlap. This color change can provide a semi-quantitative measure. For example, if the co-localized area stains blue, it indicates that the antigen indicated by Emerald is expressed at higher concentrations in the cell. Conversely, if the color appears purple, it suggests that the antigen indicated by Permanent-Red is present at higher concentrations. Additionally, the kit employs a non-biotin system to prevent nonspecific binding of endogenous biotin.