Biomarker Colocalization Imaging Analysis

Biomarker Colocalization Imaging Analysis

Tissue biomarkers are playing an increasing role in scientific research, disease diagnosis and treatment response prediction. The scope of histology or tissue biomarker identification can range from conventional histochemistry (such as H&E) to nucleic acid (ie, DNA, RNA) and protein detection. Although traditional histochemical staining is still the most widely used method, immunohistochemistry (IHC) has become the most commonly used technique for the specific detection of tissue biomarkers.

Example of estrogen receptor quantificationFigure 1.Example of estrogen receptor quantification (Lara, et al. 2021).

Biomarker Colocalization Imaging Analysis

The combination of immunohistochemistry (IHC) and fluorescence microscope provides an important and widely used tool for researchers and pathologists to image and analyze a variety of biomarkers in tissue samples. Researchers and pathologists of CD BioSciences determine the range and changes of cell populations by immunohistochemistry, and check the presence of indicated receptors and markers in diseases, so as to evaluate the effects of drug treatment on animal and human tissues.

Analysis method Brightfield imaging
Sample requirement Whole tissue fixed and stored in PBS with 0.05% azide
Formalin fixed paraffin embedded (FFPE) tissue blocks
Tissues embedded in OCT
Pre-stained and mounted slides
Image analysis Cell counting
Colocalization analysis
Nearest neighbor analysis

Biomarker Colocalization Imaging Analysis Workflow

The following is the general process of biomarker colocalization imaging analysis:

Step 1

Sample preparation

Tissue samples are transferred to PBS w/0.05% azide or made into FFPE, OCT compounds.

Step 2

Sample staining

Use immunohistochemical techniques to process, section, and stain samples.

Step 3


The sample slide is imaged under a high-throughput slide scanner.

Step 4


Analyze the images according to customer requirements.

Step 5

Send data

Send images and quantitative reports to customers.


Whole slide images in RGB format
Statistical analysis
Other relevant data

Data tables containing cell counts or area for biomarkers
Histograms of spatial distribution for nearest neighbor analysis

Our Advantages

  • A large number of tissue sections can be imaged and analyzed in a short time
  • Simultaneous detection of multiple biomarkers
  • Provide reliable and accurate test results
  • Experienced scientists provide experimental consultation
  • Reasonable price and short turnaround time

CD BioSciences has researchers with rich experience in the field of pathological imaging analysis, who can skillfully use imaging equipment and image analysis software. We can provide you with colocalization evaluation and quantitative analysis services of important biomarkers in disease pathology. If you have any needs, please feel free to contact us.

  1. Van Eycke, Yves-Rémi, et al. "High-throughput analysis of tissue-based biomarkers in digital pathology." 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). IEEE, 2015.
  2. Lara, Haydee, et al. "Quantitative image analysis for tissue biomarker use: a white paper from the digital pathology association." Applied Immunohistochemistry & Molecular Morphology 29.7 (2021): 479.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

Please note: Our services can only be used for research purposes. Do not use in diagnostic or therapeutic procedures!

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